EMBRACING SKIN
ELASTICITY AND WELLBEING

NATURAL INGREDIENTS
HELP TO PREVENT AND REPAIR SKIN DAMAGE CAUSED
BY SENILITY AND POLLUTION

ENVIRONMENTAL
POLLUTANTS
PM 10 / PM 2.5 / HYDROCARBONS






Environmental pollution can have a significant impact on skin aging.
Exposure to air pollution can promote aging and inflammation of the
skin, leading to premature skin aging. The pollution particles are small
enough to enter the skin and can accelerate brown spots,
fine lines, and wrinkles.
The skin is the main barrier that protects us against environmental
stressors, and these stressors, combined with internal factors, are re-
sponsible for cutaneous aging. Environmental factors that influence
skin aging and carcinogenesis fall into the following major catego-
ries: sun radiation, pollution, and other environmental stressors


As we age, our skin loses elasticity, which can lead to sagging and wrinkles.
The loss of elasticity in the skin is due to a decrease in collagen and elastin
production, which are essential proteins for skin structure and elasticity.
studies suggest that the skin begins to lose elasticity and thickness in the
mid-20s to early 30s. the skin also becomes thinner, more fragile, and loses
some of its protective fat layer, making it more susceptible to cell DNA
damage. The skin undergoes changes that reduce defenses against skin
disease, including weaker immune systems and poorer healing capacity.
Thinner skin also allows UV light to penetrate more deeply which leads to
the risk of developing sunlight-induced skin cancer increases. Therefore, it is
important to protect the skin from aging and environmental stressors
through Three mechanisms: Protection against ROS, Enhance collagen re-
generation, and Protect DNA.


PREVENTS AND REPAIRS SKIN DAMAGE CAUSED BY SENILITY AND POLLUTION



NATURAL INGREDIENTS
True natural derived concentrate extraction from Black Galingale, native to Southeast
Asia and Thailand. Thai indigenous knowledge prepare Black Galingale for health
Rejuvenation, especially in men. SNPS Implemented fully traceable system to track
down the safety and quality of the raw materials from Phitsanulok, Phetchabun, Nan,
Loei, Tak, Kanchanaburi, and mostly in the northern part of Thailand. Specialty Natural
Products conducts intensive research and development of this precious herb for
cosmeceuticals, functional food and medical uses.



UNIQUE BIOACTIVE COMPOUNDS
BGOLD is the purest form of unique bioactive compounds including flavoneand 5,7-Dimethoxyflavones that was extracted and purified through greenextraction technology. With innovation extraction process, we can remove dark color, impurities and toxins.

EFFECTIVE MODE OF ACTIONS
BGOLD embraces healthy skin with Three mechanism enhances collagen type I synthesis, reduced
photo-induced 8-Oxo-2′-deoxyguanosine production and inhibited photo-induced ROS production,
leaving the healthy skin youthful. BGOLD is recommended for intensive anti-wrinkle, anti-aging, and
anti-oxidant cosmeceuticals. Flavonoids have a special function to inhibit the activity of prematurely aging
cells, obstruct the secretion of inflammatory proteins that cause premature aging, and also stimulate the
collagen in our skin, and maintain skin damage from UV.
IN VITRO EFFICACY EVALUATION 1 :
COLLAGEN REGENERATION
TITLE
Effects of BGOLD on Collagen Type I induction
METHOD
3D skin model are tested with substances and samples. as below, 1 time per day and UVA/UVB irradiation 1 time per day for 5 days.
- BGOLD 1 mg/ml
- BGOLD 2 mg/ml
DMSO (vehicle control)
Retinol (positive control) 5 mg/ml
After the above treatments, 3D skin model were analyzed for collagen type I
by immunofluorescence staining technique
KEY FINDINGS
BGOLD 1, 2 mg/ml enhanced collagen type I synthesis by 48% in a photoaged 3D skin model compared to control (DMSO). In conclusion, BGold has the effect of stimulating the production of collagen type I.

BGOLD enhanced collagen type I synthesis by 48-50% in photoaged 3D skin model compared to control)
FIGURE 1
IMPACT OF BGOLD ON SKIN COLLAGEN REGENERATION.

DATA SHOWS SIGNIFICANT INCREASE OF COLLAGEN TYPE 1 SYNTHESIS IN BGOLD 1MG/ML AND 100 2MG/ML (INTEGRATED DENSITY)
IMAGE ON 3D SKIN MODEL POST TREATMENT SHOWS
SIGNIFICANT HIGHER AMOUNT OF COLLAGEN SYNTHESIS

NUCLEUS (BLUE), COLLAGEN TYPE I (GREEN)
IN VITRO EFFICACY EVALUATION 2 :
INHIBITION OF DNA DAMAGE
TITLE
Effects of BGOLD on protects against DNA damage from UVA/UVB undertested conditions
METHOD
3D skin model are tested with substances and samples. as below, 1 time per day and UVA/UVB irradiation 1 time per day for 5 days.
- DMSO (vehicle control)
- Retinol (positive control) 5 mg/ml
- BGOLD 1 mg/ml
- BGOLD 2 mg/ml
After the treatments, collected medium culture for measurement of 8-hy-droxy-2′-deoxyguanosine (8-OHdG) by ELISA technique
KEY FINDINGS
BGOLD 1, 2 mg/ml reduced photo-induced 8-hydroxy-2′-deoxyguanosine production by 44.6% in photoaged 3D skin model compared to the control. In conclusion, BGold can protect against DNA damage from UVA/UVB.

BGOLD reduced photo-induced 8-Oxo-2'-deoxyguanosine production by 44.6% and 36.3%, respectively in photoaged 3D skin model compared to control
FIGURE 2
IMPACT OF BGOLD ON INHIBITION OF DNA DAMAGE

IN VITRO EFFICACY EVALUATION 3 :
INHIBITION OF PHOTO-INDUCED ROS
TITLE
Effects of BGOLD on anti-oxidant activity from UVA/UVB
METHOD
3D skin model are tested with substances and samples. as below, 1 time per day and UVA/UVB irradiation 1 time per day for 5 days.
- DMSO (vehicle control)
- Retinol (positive control) 5 mg/ml
- BGOLD 1 mg/ml
- BGOLD 2 mg/ml
After the above treatments, 3D skin model were analyzed for Reactive
Oxygen Species (ROS).
KEY FINDINGS
BGOLD 1, 2 mg/ml inhibited photo-induced ROS production by 35% in pho – toaged 3D skin model compared to the control. In conclusion, BGold is
qualified for anti-oxidant activity.

BGOLD inhibited photo-induced ROS production by 35.3% and 56.9%, respectively in photoaged 3D skin model compared to control.)
FIGURE 3
TITLE
IMPACT OF BGOLD ON ANTI-OXIDANT ACTIVITY


CLINICAL STUDY
(IN-VIVO TEST)
IN VIVO EFFICACY TEST 1 :
ANTI-WRINKLE
TITLE
Effects of BGOLD” on Anti-Wrinkle
METHOD
Volunteers : 20 women aged 30-50
Area : Forehead and Eye
Placebo : Base cream without active ingredient
Active : Base cream with 3% BGOLD”
RESULTS ANTI-WRINKLE TEST BGOLD" 3 PERCENT IN FORMULA

KEY FINDINGS
A STUDY INVOLVING 20 FEMALE PARTICIPANTS AGED 30-50 EVALUATED THE EFFICACY OF A CREAM CONTAINING 3% BGOLD , APPLIED TWICE DAILY FOR 14 AND 28 DAYS. THE RESULTS DEMONSTRATED SIGNIFICANT WRINKLE REDUCTION ON BOTH THEFOREHEAD AND AROUND THE EYES WHEN COMPARED TO A PLACEBO

- Forehead wrinkles were reduced by 9.69% atter 14 days and by 17.76% after 28 days.
- Eye wrinkles were reduced by 8.16% after 14 days and by 17.04% after 28 days.
IN VIVO EFFICACY TEST 2 :
SKIN SMOOTHNESS
TITLE
Effects of BGOLD on Skin Smoothness
METHOD
Volunteers : 20 women aged 30-50
Area : Forehead and Eye
Placebo : Base cream without active ingredient
Active : Base cream with 3% BGOLD”
RESULTS ANTI-WRINKLE TEST BGOLD 3 PERCENT IN FORMULA

KEY FINDINGS
A STUDY INVOLVING 20 FEMALE PARTICIPANTS AGED 30-50 EVALUATED THE EFFICACYE OF A CREAM CONTAINING 3% BGOLD”, APPLIED TWICE DAILY FOR 14 AND 28 DAYS. RESULTSDEMONSTRATED A SIGNIFICANT INCREASE IN SMOOTHNESS ON BOTH THE FOREHEAD AND AROUND THE EYES COMPARED TO A PLACEBO:

- Forehead wrinkles were reduced by 9.69% atter 14 days and by 17.76% after 28 days.
- Eye wrinkles were reduced by 8.16% after 14 days and by 17.04% after 28 days.
CLINICAL EVALUATION
20 INDIAN (FEMALE) HEALTHY HUMAN SUBJECTSSPECIAI
28 DAYS FOLLOWING THE FIRST APPLICATION OF PRODUCT.
DERMATOLOGICAL EVALUATION: EFFICACY (FACE SERUM: WITH B-GOLD (FSBG:LD-01) : Product A 0.2 % cream base
DERMATOLOGICAL EVALUATION: FACE SERUM: WITH &-GOLD (FSBG:MD-03) :Product B 1 % cream base






CORNEOMETRY ( PRODUCT A) A SIGNIFICANT INCREASE IN THE CAPACITANCE PARAMETER BY 74.61% ON THE WHOLE FACE TREATED WITH TEST PRODUCT A ON AVERAGE ON WHOLE PANEL AT T+28 DAYS, SHOWS A MOISTURIZING EFFECT OF THE PRODUCT
CORNEOMETRY ( PRODUCT B) A SIGNIFICANT INCREASE IN THE CAPACITANCE PARAMETER BY 83.95% ON THE WHOLE FACE TREATED WITH TEST PRODUCT B ON AVERAGE ON WHOLE PANEL AT T+28 DAYS, SHOWS A MOISTURIZING EFFECT OF THE PRODUCT.
CUTOMETRY ( PRODUCT A ) A SIGNIFICANT DECREASE IN THE RO PARAMETER BY 3.4% ON THE WHOLE FACE TREATED WITH TEST PRODUCT A ON AVERAGE ON WHOLE PANEL AT T+28 DAYS SHOWS AN EFFECT OF THE PRODUCT IN TERMS OF SKIN FIRMNESS.
CUTOMETRY (PRODUCT B) A SIGNIFICANT DECREASE IN THE RO PARAMETER BY 3.9% ON THE WHOLE FACE TREATED WITH TEST PRODUCT B ON AVERAGE ON WHOLE PANEL AT T+28 DAYS SHOWS AN EFFECT OF THE PRODUCT IN TERMS OF SKIN FIRMNESS
TEWAMETRY ( PRODUCT A) A SIGNINCANT DECREASE IN THE TEWL PARAMETER BY 16.53% ON THE WHOLE FACE TREATED WITH TEST PRODUCT A ON AVERAGE ON WHOLE PANEL AT T+28 DAYS SHOWS A PREVENTION OF WATER LOSS FROM SKIN .E. IMPROVEMENT IN SKIN WATER BARRIEER FUNCTION.
TEWAMETRY ( PRODUCT B) A SIGNIF CANT DECREASE IN THE TEWL PARAMETER BY 17.14% ON THE WHOLE FACE TREATED WITH TEST PRODUCT B ON AVERAGE ON WHOLE PANEL AT T+28 DAYS SHOWS A PREVENTION OF WATER LOSS FROM SKIN I.E. IMPROVEMENT IN SKIN WATER BARRIER FUNCTION.

SPECIFICATION (LIQUID)
PRODUCT CODE : BI-EL11-KAP
TYPICAL SPECIFICATION
APPEARANCE
HEAVY METALS
ARSENIC
LEAD
PH DIRECT
SPECIFIC GRAVITY (D20)
TOTAL AEROBIC COUNT20
YEAST & MOLD
ENTEROBACTERIA COUNT
S. AUREUS
P. AERUGINOSA
E. COLI
SALMONELLA SPP.
CONTENT OF ACTIVE INGREDIENTS
Yellow liquid
Not more than 20 ppm
Not more than 2 ppm
Not more than 1 ppm
6.00-8.50
1.00-1.20
Not more than 1,000 cfu/g
Not more than 100 cfu/g
Not more than 100 cfu/g
Absent
Absent
Absent
Absent
Not less than 500.0 mg. %
5,7-dimethoxyflavone (DMF)
WE OFFER STANDARDIZED THAI HERBAL EXTRACTS

